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. 2021 Jul 31;12(8):758. doi: 10.1038/s41419-021-04040-9

Fig. 4. FBXO16 interacts with hnRNPL.

Fig. 4

A The interaction protein network of FBXO16 revealed by the BioGRID database (https://thebiogrid.org/). B The interaction proteins of 18 F-box proteins were collected by the BioGRID database. Red represents positive hit. Blue represents negative hit. C The cell lysates from HEK293T cells stable expressing empty vector (EV) or FLAG-FBXO16 were subjected to immunoprecipitation (IP) with FLAG M2 beads, followed by immunoblotting with indicated antibodies. D A2078 cells treated with 10 µM MG132 for 4 h were lysed and cell lysate was subjected to IP assay with anti-hnRNPL antibody, followed by immunoblotting with anti-FBXO16 antibodies. E IP assay was performed to detect the interaction between the endogenous FBXO16 and hnRNPL in OVCAR8 cells treated with MG132. F Schematic diagram of the different FLAG-tagged FBXO16 deletion mutants. G Cell lysates of HEK293T cells transiently expressing either full-length FLAG-FBXO16 or FLAG-FBXO16 mutants were immunoprecipitated with FLAG M2 beads, and immune complexes were blotted with anti-hnRNPL antibody. 293T cells transfected HA-hnRNPL plasmid were lysed and incubated with GST alone or GST-FBXO16 immobilized on GST-Sepharose beads. The bound proteins were then detected by immunoblotting with indicated antibodies. H Schematic diagram of the different FLAG-tagged hnRNPL deletion mutants. I Cell lysates of HEK293T cells transiently expressing either full-length FLAG-hnRNPL (1-583) or FLAG-hnRNPL mutants were immunoprecipitated with FLAG M2 beads, and the immune complexes were blotted with anti-FBXO16 antibody.