Fig. 1. Overview of experimental methods.

MR guided FUS was applied at either 0.2 MPa or 0.4 MPa to mouse striata following IV injection of mRuby plasmid conjugated to cationic MB. Brains were excised and dissociated, producing single-cell suspensions containing both untransfected and transfected cells. Using cells from the control condition to define the mRuby gating strategy, mRuby+ cells were sorted from FUS-treated brains by FACS. Single-cell RNA-sequencing was performed on untransfected, untreated cells from the control condition, mRuby+ cells from the 0.2 MPa condition, and mRuby+ cells from the 0.4 MPa condition.