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. 2021 Feb 1;26(9):5371–5388. doi: 10.1038/s41380-021-01034-z

Fig. 5. co-IP of MAP2 from mouse brain and functional effects of S1782E on protein synthesis.

Fig. 5

a Using conservative cutoff criteria of q < 0.05, 6095 peptides (red) from 590 proteins were identified as potential MAP2 interactors. b Puromycin incorporation was detected via western blotting following a 1 h treatment of transiently transfected HEK293 cells expressing MAP2c-WT or MAP2c-S426E in comparison to cells transfected with eGFP control for 48 h. Densitometric quantification of relative puromycin incorporation normalized to MAP2 expression levels. N = 4, data shown are average across experiments ± SEM. *p < 0.1, **p < 0.05. c Reductions in the synaptic proteome are seen in both MAP-IRLOW Sz and S1782E mice. Volcano plots showing distribution of 159 synaptically enriched proteins in NPC, MAP2-IRLOW, and MAP2-IRNL Sz (N = 45 pairs; N = 19 MAP2-IRNL, N = 26 MAP2-IRLOW). Mice with phosphomimetic mutation S1782E have synaptic proteome alterations similar to those seen in MAP2-IRLOW Sz (N = 4 mice/genotype). Green dashed line indicates q-value of 0.05, red dashed line indicates q-value of 0.1.