Fig. 4.

Effect of RNase treatment on the anti-inflammatory activity of MSC-IL-Exo. A RNA profiles of MSC-Exo and MSC-IL-Exo are displayed up to 300 nt in the histograms. The peak at 4 nt is a low size marker (arrowhead). B MSC-IL-Exo was treated with 10 U/mL RNase (Ambion) for 3 h at 37 °C to prepare the sample without RNA content (RNase-IL-Exo). SW982 cells were treated with IL-1β (10 ng/mL) and TNF-α (25 ng/mL) for 24 h in the presence of 5 μg/mL of MSC-IL-Exo or RNase-IL-Exo. mRNA levels of selected pro-inflammatory cytokines (IL-1β, IL-6, and MCP-1) were analyzed via RT-qPCR. Values are normalized by that of GAPDH and presented as mean ± SD from three independent experiments (n = 3). *p < 0.05, ^##/**p < 0.01, and ^###p < 0.001 by one-way ANOVA for two exosome groups (MSC-Exo and MSC-IL-Exo) versus untreated control (^#) or versus IL-1β and TFN-α groups (*)