Fig. 5.

Role of miRNAs in the anti-inflammatory activity of MSC-IL-Exo. A Levels of miR-143, miR-146, miR-155, and miR-147b were examined in MSC-Exo and MSC-IL-Exo via RT-qPCR analysis. ***p < 0.001 between two groups by one-way ANOVA. B SW982 cells were transfected with synthetic miR-147b mimics at 0, 20, and 50 nM. The amount of miR-147b was examined via RT-qPCR analysis. **p < 0.01 and ***p < 0.001 versus the control group by one-way ANOVA. C SW982 cells were treated with IL-1β (10 ng/mL) and TNF-α (25 ng/mL) for 24 h in the presence of 10 μg/mL MSC-IL-Exo or after transfection of miR-147b mimics or miR-147b inhibitor. mRNA levels of selected pro-inflammatory cytokines (IL-1β, IL-6, and MCP-1) were analyzed by RT-qPCR. ^#/*p < 0.05, ^##/**p < 0.01, and ^###/***p < 0.001 for three treatment groups (MSC-IL-Exo, miR-147b mimics, and miR-147b inhibitor) versus untreated control (^#) or versus IL-1β and TFN-α groups (*) by one-way ANOVA. All values are normalized by that of GAPDH and presented as mean ± SD from three independent experiments (n = 3)