Table 1.
Primers | Sequences | Length (bp) | Annealing temp. (°C) |
---|---|---|---|
GAPDH |
F: 5′-TGCACCACCAACTGCTTAGC-3′ R: 5′-GGCATGGACTGTGGTCATGAG-3′ |
87 | 60 |
IL-1β |
F: 5′-ACAAGGAGAAGAAAGTAATGAC-3′ R: 5′-GCTGTAGAGTGGGCTTAT-3′ |
104 | 60 |
IL-6 |
F: 5′-CACCTCAGATTGTTGTTGTT-3′ R: 5′-AATAGTGTCCTAACGCTCAT-3′ |
119 | 60 |
MCP-1 (CCL2) |
F: 5′-CCAGTCACCTGCTGTTAT-3′ R: 5′-GCTTCTTTGGGACACTTG-3′ |
98 | 60 |
SOCS1 |
F: 5′-CACCTCCTACCTCTTCAT-3′ R: 5′-AATAAAGCCAGAGACCCT-3′ |
80 | 60 |
SOCS3 |
F: 5′-AGACGGGACATCTTTCAC-3′ R: 5′-CGAATCTCTTAGCCAGACATA-3′ |
89 | 60 |