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. 2021 Jan 25;18(4):525–536. doi: 10.1007/s13770-020-00324-x

Table 1.

Primers used in RT-qPCR after the Reverse Transcription of mRNAs

Primers Sequences Length (bp) Annealing temp. (°C)
GAPDH

F: 5′-TGCACCACCAACTGCTTAGC-3′

R: 5′-GGCATGGACTGTGGTCATGAG-3′

87 60
IL-1β

F: 5′-ACAAGGAGAAGAAAGTAATGAC-3′

R: 5′-GCTGTAGAGTGGGCTTAT-3′

104 60
IL-6

F: 5′-CACCTCAGATTGTTGTTGTT-3′

R: 5′-AATAGTGTCCTAACGCTCAT-3′

119 60
MCP-1 (CCL2)

F: 5′-CCAGTCACCTGCTGTTAT-3′

R: 5′-GCTTCTTTGGGACACTTG-3′

98 60
SOCS1

F: 5′-CACCTCCTACCTCTTCAT-3′

R: 5′-AATAAAGCCAGAGACCCT-3′

80 60
SOCS3

F: 5′-AGACGGGACATCTTTCAC-3′

R: 5′-CGAATCTCTTAGCCAGACATA-3′

89 60