Fig. 4.
ADAR1K999N mutation causes RNA editing changes on neuron 5HTR2c mRNA substrate. The RNA editing activities of the K999N mutant protein in the mouse brain on 5HTR2c mRNAs were assessed by sequencing analysis. RNAs from whole brain tissues were amplified by PCR with specific primers for 5HTR2c following reverse transcription, and the PCR products were subject to Sanger sequencing analysis. A The editing levels of 5HTR2c mRNA at A, B, C, D, and E sites in ADAR1K999N and wild type control mice were shown by the representative chromatograms of brain RNA Sanger sequencing. The editing sites are marked by the arrows. B The editing level on each editing site was compared between ADAR1 K999N mutant (n = 5) and wild type (n = 4) mice. Editing on A, B, and C sites in ADAR1K999N mice was significantly decreased. However, editing on the D site was increased by the mutation. p < 0.05 for A, B, C and D sites. Editing at the E site occurred at baseline low efficiencies, with no differences in editing efficiency between the ADAR1K999N mice and WT controls. The Wilcoxon rank-sum test was used to determine the statistical difference between ADAR1K999N mice and WT controls