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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Inflammation. 2021 Feb 2;44(3):1130–1144. doi: 10.1007/s10753-020-01408-5

Fig. 2.

Fig. 2.

FAK inhibition decreases IKK activation and activity in endothelial cells. a–c HAoECs were treated for 1 h with DMSO or PF-271 (2.5 μmol/L) prior to TNF-α (10 ng/mL) stimulation for the indicated times. a Western blot analysis of pY397 FAK, FAK, pS176/177 IKKα/β, IKKα/β, IκBα, and GAPDH (n = 3). b FAK localization (green; mouse) and active pS176/177 IKKα/β (red; rabbit) were evaluated by immunostaining (n = 3). Scale bar, 20 μm. c IKKβ was immunoprecipitated (IP) from stimulated HAoECs and in vitro IKK assay was performed. Western blot analysis of IKKβ, pS32/36 IκBα, and GST-IκBα in IKK kinase assay and pY397 FAK, FAK, and β-actin of inputs (n = 3).