Table 1.
Antibodies Used in This Study
| Name of antibody | Manufacturer, catalog# | Species raised in | Dilution used | RRID |
|---|---|---|---|---|
| pY397 FAK | Thermo Fisher Scientific, 44-624G | Rabbit | 1:3000 (WB) 1:300 (IF) |
AB_2533701 |
| pS32/36 IκBα | Thermo Fisher Scientific, MA5-15224 | Mouse | 1:2000 (WB) | AB_10981266 |
| NF-κB p65 | Thermo Fisher Scientific, 51-0500 | Rabbit | 2 μg (ChIP) | AB_2533893 |
| pS176/177 IKKα/β | Thermo Fisher Scientific, MA5-14857 | Mouse | 1:100 (IF) | AB_10986824 |
| Alexa 488 anti-mouse | Thermo Fisher Scientific, A11001 | Goat | 1:1000 (IF) | AB_2534069 |
| Alexa 594 anti-mouse | Thermo Fisher Scientific, A11005 | Goat | 1:1000 (IF) | AB_2534073 |
| Alexa 488 anti-rabbit | Thermo Fisher Scientific, A11008 | Goat | 1:1000 (IF) | AB_143165 |
| Alexa 594 anti-rabbit | Thermo Fisher Scientific, A11012 | Goat | 1:1000 (IF) | AB_2534079 |
| Anti-mouse HRP | Thermo Fisher Scientific, 31430 | Goat | 1:5000 (WB) | AB_228307 |
| Anti-rabbit HRP | Thermo Fisher Scientific, 31460 | Goat | 1:5000 (WB) | AB_228341 |
| VCAM-1 | Santa Cruz Biotechnology, sc-8304 | Rabbit | 1:2000 (WB) 1:200 (IF) |
AB_2214058 |
| pS311NF-κB | Santa Cruz Biotechnology, sc-33039 | Rabbit | 1:2000 (WB) | AB_2238379 |
| pS529 NF-κB | Santa Cruz Biotechnology, sc-101751 | Rabbit | 1:2000 (WB) | AB_1128538 |
| NF-κB | Santa Cruz Biotechnology, sc-372 | Rabbit | 1:2000 (WB) 1:200 (IF) |
AB_632037 |
| IκBα | Santa Cruz Biotechnology, sc-371 | Rabbit | 1:2000 (WB) | AB_2235952 |
| Src | Santa Cruz Biotechnology, sc-18 | Rabbit | 1:2000 (WB) | AB_631324 |
| vWF | Santa Cruz Biotechnology, sc-271409 | Mouse | 1:200 (IF) | AB_10610681 |
| RIPK1 | Santa Cruz Biotechnology, sc-133102 | Mouse | 1:2000 (WB) | AB_1568814 |
| NEMO | Santa Cruz Biotechnology, sc-8032 | Mouse | 1:2000 (WB) | AB_627786 |
| TNFR1 | Santa Cruz Biotechnology, sc-374186 | Mouse | 1:200 (IF) | AB_10992436 |
| β-actin | Sigma-Aldrich, A5316 | Mouse | 1:5000 (WB) | AB_476743 |
| FLAG | Sigma-Aldrich, F3165 | Mouse | 1:2000 (WB) 2 μg (IP) |
AB_259529 |
| FAK | Millipore, 05–537 | Mouse | 1:2000 (WB) 1:300 (IF) |
AB_2173817 |
| GAPDH | Millipore, MAB374 | Mouse | 1:5000 (WB) | AB_2107445 |
| TNFR1 | Novus Biologicals, NBP1-97453 | Rabbit | 1:2000 (WB) 1:200 (IF) |
AB_11188877 |
| pS176/177 IKKα/β | Cell Signal Technology, 2697 | Rabbit | 1:2000 (WB) 1:200 (IF) |
AB_2079382 |
| pS536 NF-κB | Cell Signal Technology, 3033 | Rabbit | 1:2000 (WB) 1:200 (IF) |
AB_331284 |
| RIPK1 | Cell Signal Technology, 4926 | Rabbit | 1:200 (IF) | AB_2224503 |
| pY418 Src | Cell Signal Technology, 2101 | Rabbit | 1:2000 (WB) | AB_331697 |
| IKKα/β | Bethyl Laboratories, A301-827A | Rabbit | 2 μg (IP) | AB_1264322 |
| vWF | Agilent Technologies, A0082 | Rabbit | 1:500 (IF) | AB_2315602 |
| RIPK1 | BD Biosciences, 610459 | Mouse | 1:200 (IF) | AB_397832 |
| GST | In-house against full-length recombinant GST (Thermo Fisher Pierce Antibodies) | Rabbit | 1:2000 (WB) | N/A |
| FAK | In-house against amino acids 896–912 of human FAK (DSYNEGVKLQPQEISPP; GenScript) | Rabbit | 1:500 (IF) | N/A |
RRID research resource identifier, WB western blot, IF immunofluorescence, ChIP chromatin immunoprecipitation, IP immunoprecipitation