Figure 2.

C. tropicalis promotes the chemotherapy resistance in SW480 cells. SW480 cells were treated with oxaliplatin in the presence or absence of C. tropicalis. (A) Cell viability was detected by using CCK8 assay. (B) The EC50 value in different SW480 cells was calculated. (C-D) Proportion of apoptotic cells was detected by flow cytometry. (E-F) Cleaved caspases 3(CC3) and 9 (CC9) expression were detected by western blot. Data with error bars are represented as mean ± SD. Each panel is a representative experiment of at least three independent biological replicates. *p < 0.05, **p < 0.01 and ***p<0.001as determined by unpaired Student's t test.