Figure 3.

MMR is inhibited upon C. tropicalis treatment. (A-C) Mice were treated as described in Figure S1A. Tumors were acquired. Tumor tissues were stained for MLH1 and MSH2. The percentages of MLH1 and MSH2-positive tumor cells were quantified (A). mRNA expression of MLH1 and MSH2 in tumor tissues were detected by qPCR (B). Protein expression of MLH1 and MSH2 were detected by western blot (C). (D-F) SW480 cells were treated with oxaliplatin in the presence or absence of C. tropicalis. mRNA expression of MLH1 and MSH2 in cells were detected by qPCR (D). Protein levels of MLH1 and MSH2 were detected by western blot (E). p-H2AX expression were detected by immunofluorescence (F). Data with error bars are represented as mean ± SD. Each panel is a representative experiment of at least three independent biological replicates. Magnifications are 40× (scale bar, 0.05 mm) *p < 0.05, **p < 0.01 and ***p<0.001 as determined by unpaired Student's t test.