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. 2021 Jul 5;17(11):2795–2810. doi: 10.7150/ijbs.60509

Figure 6.

Figure 6

miR-21a-5p inhibits the effect of CNTF by inhibiting the expression of Cntfr α. Three types of Cntfr α siRNA were transfected into astrocytes. The expression of Cntfr α mRNA was detected by qRT-PCR (A). The expression of CNTFR α protein was detected by western blotting, and the results were analyzed with Image J, GraphPad, and SPSS (B). (C) miR-21 inhibitor, Cntfr α siRNA, and negative controls were transfected into astrocytes, and the expression of Cntfr α mRNA was detected by qRT-PCR, normalized to GAPDH. (D-G) miR-21 inhibitor, Cntfr α siRNA, and negative controls were transfected into astrocytes. After pre-treating with CNTF, astrocytes were induced into A1s. The expression of C3, Serping1, H2D1, and S100a10 mRNA were detected by qRT-PCR and normalized to GAPDH. The results were analyzed with GraphPad and SPSS. The data are expressed as mean ±SD, n=3. *p <0.05, **p <0.01, ***p <0.001. (H-I) Immunofluorescence was used to detect GFAP (green; H-I), C3 (red; H), S100a10 (red; I) and DAPI (blue; nuclei), n=3; the results were analyzed with Image J, GraphPad, and SPSS (J). *p <0.05, **p <0.01, ***p <0.001.