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. 2021 Jun 26;17(11):2683–2702. doi: 10.7150/ijbs.61350

Figure 3.

Figure 3

The binding site of NF-κB p65 plays a critical role in TNF-α mediated activation of CXCL10 promoter. (A) SW480/SW620 cells were transfected with Renilla luciferase and pNF-κB-Luc plasmids. Cells were lysed after 24 h of TNF-α (0-20 ng/ml) treatment, followed by measurement of luciferase activity. (B) pCXCL10-Luc (-250/+8) promoter plasmid and NF-κB-p65 (RelA) expression plasmid was transfected together or separately into SW480/SW620 cells. Cells were lysed after 24 h of TNF-α (0-20 ng/ml) treatment, followed by measurement of luciferase activity. (C) pCXCL10-Luc (-1000/+8), pCXCL10-Luc (-250/+8), or pCXCL10-Luc (-500/+8) promoter plasmid and NF-κB-p65 expression plasmid was transfected separately along with the Renilla luciferase plasmid into SW480/SW620 cells. Cells were lysed after 24 h of TNF-α (20 ng/ml) treatment, followed by measurement of luciferase activity. (D) Wild-type pCXCL10-Luc (-250/+8) or p65 binding site mutant pCXCL10 mtRelA (p65)-Luc (-250/+8) promoter plasmid was transfected separately into SW480/SW620 cells along with the Renilla luciferase plasmid. Cells were lysed after 24 h of TNF-α (20 ng/ml) treatment, followed by measurement of luciferase activity. In these experiments, all fold induction data were calculated by dividing the firefly luciferase luminescence intensity by that of Renilla luciferase. Every bar chart stands for mean ±SD (n=3). *p < 0.05, **p < 0.01, ns: not significant by the Sidak multiple comparison test.