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. 2021 Jun 26;17(11):2683–2702. doi: 10.7150/ijbs.61350

Figure 4.

Figure 4

CXCL10 regulates CC cell colony formation, invasion, and migration. siNC or siCXCR3 was transfected into SW480/SW620 cells. Wound healing (A) and Transwell (B) assays were carried out in SW480, siNC-SW480, siCXCR3-SW480, SW620, siNC-SW620 and siCXCR3-SW620 cells after treatment with or without CXCL10 (200 ng/ml). The wound closure percentage was calculated: magnification 200×; scale bar=200 μm. Invading cell number was determined: magnification 50×; scale bar=50 μm. (C) Vector plasmid (Vector) or overexpressing CXCL10 plasmid (CXCL10) was transfected into SW480/SW620 cells for clone formation experiments. (D) CXCL10 (200 ng/ml) was used to stimulate SW480/SW620 cells to lyse total proteins at specific time points, the activated GTP-RhoA was bound to GST-Rhotekin-RBD fusion protein, and the activated GTP-cdc42 was bound to GST-PAK1-PBD fusion protein. Glutathione resin was used for the immunoprecipitation of GTP-bound cdc42 and GTP-bound RhoA. The GTP-RhoA and GTP-cdc42 activation was detected by WB assay. The band intensities of RhoA and cdc42 relative to those of the input were measured using Image J software. Every bar chart stands for mean ±SD (n=3). *p < 0.05, **p < 0.01 by Sidak multiple comparison test.