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. 2021 Jun 26;17(11):2683–2702. doi: 10.7150/ijbs.61350

Figure 6.

Figure 6

CXCL10 regulates EMT in CC cells through the PI3K/Akt/GSK-3β/Snail pathway. (A) CXCL10 (200 ng/ml) was used to stimulate SW480/SW620 cells for 4 days, and protein levels of Slug, Snail, ZEB1 and Twist were measured through WB assays. (B) CXCL10 (0-200 ng/ml) was used to treat SW620 cells for 4 days, then Snail protein level was examined through WB assays. (C) siSnail or siNC was transfected into SW620 cells, followed by 4 days of stimulation with CXCL10 (200 ng/ml). E-cad, N-cad and Vimentin protein expression was evaluated through WB assays. (D) CXCL10 (200 ng/ml) was used to stimulate SW620 cells at specified time point. p-Akt/Akt, p-PI3K/PI3K and p-GSK-3β/GSK-3β levels were detected by WB assay. (E-F) LY294002 (10 mM) was used to treat SW620 cells for 1 h before CXCL10 (200 ng/ml) stimulation, the Snail, Vimentin, N-cad, E-cad, p-Akt/Akt, and p-GSK-3β/GSK-3β expression was measured through WB assays. (G-H) SW620 cells were pretreated with CHIR-99021 (40 mM) for 1 h prior to CXCL10 (200 ng/ml) stimulation, the Snail, p-GSK-3β/GSK-3β, β-catenin, Vimentin, N-cad, and E-cad protein expression was measured through WB assay. (I) siGSK-3β or siNC was transfected into SW620 cells, followed by 24 h of CXCL10 (200 ng/ml) stimulation. Snail and p-GSK-3β/GSK-3β protein expression was analyzed by WB assays.