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. 2021 Jul 17;46:102070. doi: 10.1016/j.redox.2021.102070

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© 2021 The Authors. Published by Elsevier B.V.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 1 — Expression and purification of human GPX2 (A) and GPX4 (B). Lane 1, SeeBlue™ plus2 protein marker (Thermofisher catalog # LC5925). Lane 2, His and SUMO tagged GPX variants (H6SUMO-GPX2 and H6SUMO-GPX4) purified from first IMAC. Lane 3, treatment with His-tagged ULP1 on H6SUMO-GPX variant. Lane 4, proteins bound to the nickel column during second IMAC, including released H6SUMO tag, His-tagged ULP1 and other impurities eluted from first IMAC. Lane 5, flowthrough from second IMAC containing the non-tagged GPX enzymes.