Fig. 2.

Validating purified recombinant human GPX1, GPX2 and GPX4 using immunoblotting. To the left is shown the Ponceau-S staining of a nitrocellulose membrane after protein transfer from an SDS-PAGE with the purified recombinant GPX isoenzymes ran in triplicates as indicated. The membrane was subsequently cut in three parts, with each part blotted with different primary antibodies as indicated here and in the right part of the figure: Anti-GPX1 (Epitomics catalog # 3120-1), Anti-GPX2 (Abcam catalog # ab137431) and Anti-GPX4 (Abcam catalog # ab125066).