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. 2021 Jul 17;46:102070. doi: 10.1016/j.redox.2021.102070

Table 4.

Kinetic constants of recombinant human GPX preparations.

Kinetic parameters were determined with either H2O2 or PCOOH as substrates as described in detail in the Methods section.

Substrate Recombinant GPX k1 (M−1s−1) k2 (M−1s−1)
H2O2 BSP-purified GPX1 1.36 × 106 1.73 × 104
H2O2 BSP-purified GPX4 9.55 × 104 4.01 × 103
PCOOH BSP-purified GPX4 5.28 × 106 2.86 × 103