Fig. 5.

Analysis of triHOME, HODE, and epoxy-HOME EOx ceramides and free oxylipins in the healthy human epidermis. All lipids were extracted and analyzed using LC/MS/MS from human epidermal breast tissue as described in Materials and methods (n = 9, mean ± SEM). A: LA-EOx-LA ceramide lipids in the healthy epidermis. B–D: Epidermis levels of EOx oxidized ceramides in the healthy human epidermis. E–H: Free oxylipin quantification in the healthy epidermis. LA, linoleic acid; EOx, omega-hydroxy sphingosine/phytosphingosine/hydroxysphingosine.