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. 2021 Jul 26;6(7):567–580. doi: 10.1016/j.jacbts.2021.05.004

Figure 2.

Figure 2

ID Inhibits the eNOS/cGMP/PKG Pathway

Representative Western blot and quantification of (A) total eNOS and its phosphorylated form and (B) the sodium dodecyl sulfate–resistant eNOS dimer in cardiac lysates obtained from the different experimental groups, n = 5 per group. (C) Representative Western blot and quantification of total eNOS and its dimeric form in isolated cardiomyocytes treated with deferasirox (DFX), n = 4 per group. (D) Quantification of myocardial content of nitrate/nitrite (NOx) and cGMP, n = 5 per group. (E) protein kinase G (PKG) activity evaluated by Western blot as PKG-dependent phosphorylation of its substrate vasodilator-stimulated phosphoprotein (VASP), n = 5 per group. (F) Representative Western blot of inducible nitric oxide synthase (iNOS). LPS corresponds to a mouse spleen sample obtained 6 h after lipopolysaccharide injection (10 mg/kg intraperitoneally). ∗P < 0.05, ∗∗P < 0.01 vs IDD group. CD = control diet; eNOS = endothelial nitric oxide synthase; IDD = iron-deficient diet; IDD+Fe = IDD plus iron supplementation. Data are mean ± SEM.