Figure 4.

ID Reduces HSP90 Levels and Increases the Extent of eNOS Ubiquitination

(A) Representative Western blot and quantification of HSP90. (B) Polyubiquitination levels of eNOS in cardiac lysates analyzed by using agarose-tandem ubiquitin-binding entities pull-downs. eNOS bands were normalized to their corresponding ubiquitin smears and ratios were normalized to ubiquitinated eNOS level in control samples. Representative Western blots are shown in all panels. Data are mean ± SEM. ∗P < 0.05, ∗∗P < 0.01 vs IDD group. Abbreviations as in Figure 2.