Fig. 4.

Lipocalin 2 deletion in Col4a3^KO mice improves survival and prevents the development of left ventricular hypertrophy. (a) Kaplan–Meier cumulative proportion of mice surviving. Both Col4a3^KO (red) and Col4a3^KO/Lcn2^KO (coral) mice show reduced lifespan (P < 0.05 vs. WT), but Col4a3^KO/Lcn2^KO survive longer than Col4a3^KO mice (P < 0.05 vs. Col4a3^KO). (b) Heart weight to tibia length (HW/TL) ratio, and (c) left ventricular (LV) mass, (d) LV posterior wall (LVPW) thickness, (e) bright-field microscopy of hematoxylin & eosin staining (H&E) of heart cross-sections (scale bar = 1 mm), and M-mode echocardiography, and (f) ejection fraction (EF) measured in 23-week-old WT, Lcn2^KO, Col4a3^KO, and Col4a3^KO/Lcn2^KO (CPD) mice. c, d, f are calculated from echocardiography analysis. Data are presented as mean ± SE, n ≥ 10 per group, P < 0.05 vs.*WT, ^$Lcn2^KO