(a) Diffusion-weighted MRI and quantification of layers of gel containing, from top to bottom, 0.25, 0.5, 1, and 2 ×108 cells · mL−1 B16-F10 cells with 100% viability. Error bars represent one standard error. All layers have a statistically significant difference from each other (p <0.0001). (b) MT-weighted image and quantification of cell density gradient. All layers have a statistically significant difference from each other (p <0.0001). (c) Photograph of NMR tube containing cell density gradient immediately after sample prep and prior to MRI acquisition.