Figure 3.
p53 positively regulated ferroptosis caused by NDV
(A and B) Western blotting analysis of the levels of SLC7A11, GPX4, and NP after PFTα (5 μM) treatment for 24 h in NDV-infected U251 cells. β-actin was used as the loading control.
(C) Relative levels of intercellular MDA were assayed after 24 h of pretreatment with PFTα (5 μM) in U251 cells.
(D) Detection of intracellular GSH concentrations for 24 h after pretreatment with PFTα (5 μM) in U251 cells.
(E and F) Intracellular LPO in NDV-infected U251 cells treated with or without PFTα for 24 h was determined with the fluorescent probe Liperfluo (Green). Scale bars = 20 μm.
(G and H) U251 cells with stable knockdown of p53 were treated with erastin (5 μM), RSL3 (5 μM), and NDV (MOI = 5) for 24 h. Western blotting analyses were performed to determine the levels of SLC7A11, GPX4, and NP.
(I and J) Analysis of intracellular ROS levels using DCFDA staining and flow cytometry in U251 cells treated for 24 h with PFTα (5 μM).
Significance was analyzed using a two-tailed Student's t-test. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Data were expressed as mean SEM (n = 3 in each group).