Fig. 7.

GC cell-derived lactate promotes the expression of BDNF in CAFs via activation of NF-κB . A. BDNF mRNA expression in CAFs was assessed by qRT-PCR after culture in the indicated conditions. B. BDNF protein expression in the supernatants of CAFs was assessed by ELISA after culture in the indicated conditions. C. The expression of nuclear NF-κB in CAFs was detected by Western Blot in the indicated conditions. D. The nuclear translocation of p65 in CAFs was detected by IF. E. The expression of NF-κB and IkB-α in CAFs treated with indicatd concentreation of lactate were detected by Western Blot. F. BDNF mRNA expression in CAFs was assessed by qRT-PCR after culture in the indicated conditions. G. DNA fragments pulled down with NF-κB antibody in CAFs were amplified by PCR. H. Dual luciferase reporters containing four different truncations of the BDNF promoter region were co-transfected with NF-κB-expressing plasmid. The data are presented as the mean ± SD from three independent experiments (*p < 0.05; **p < 0.01).