Table 2.
Total nitrogen determination of the different extracts produced from chickpeas and peas feedstocks.
| Sample | Protein content (%DM) chickpeasa | Protein content (%DM) peasa | Protein content based on total amino acids value (% DM) chickpeasb | Protein content based on total amino acids value (% DM) peasb | Non-protein nitrogen on total nitrogen (%) chickpeasc | Non-protein nitrogen on total nitrogen (%) peasc |
|---|---|---|---|---|---|---|
| DAE (lab scale) | 74.1 ± 0.0a | 65.2 ± 0.2b | 58.0 ± 0.1 | 41.0 ± 0.3 | 22 | 35 |
| EAE (alcalase) | 37.1 ± 0.5d | 37 ± 0.7f | 28.6 ± 0.4 | 33.8 ± 0.2 | 26 | 11 |
| EAE (papain) | 46.5 ± 2.8b | 66 ± 0.1a | 35.5 ± 0.5 | 49.1 ± 0.3 | 27 | 26 |
| EAE (pepsin) | 35.8 ± 1.1d | 45 ± 0.1d | 23.5 ± 0.1 | 44.1 ± 0.1 | 37 | 2 |
| EAE (trypsin) | 35.4 ± 1.5d | 47 ± 0.8c | 25.4 ± 0.1 | 41.6 ± 0.2 | 27 | 13 |
| EAE (alcalase + papain) | 38.9 ± 0.6c | 39 ± 1.1e | 28.0 ± 0.3 | 31.0 ± 0.2 | 15 | 21 |
Equal letters mean statistically equal values (pairwise comparison of types, independent-samples median test), The number of replicates was n = 2.
a
Determined by Kjeldahl analysis.
b
Determined by sum of total AA (with the exclusion of water).
c
Determined by subtracting the amount determined in b from the amount determine in a and expressing it in % on a.