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. 2021 Jul 19;12:720387. doi: 10.3389/fphar.2021.720387

FIGURE 5.

FIGURE 5

DHT induces autophagy and reduces fibrosis by down-regulating miR-192-5p expression (A) Real-time PCR detection of miR-192-5p after HK-2 cells stimulated with HG and TGF-β1. n = 3; *p < 0.05, **p < 0.01 (B–C) Relative expression of miR-192-5p in HK-2 and NRK-49F cells transfected with miR-192-5p mimic. ***p < 0.001 (D) Relative mRNA levels of BECN1, SQSTM1, COL1A1, ACTA2, and FN1 in HK-2 cells transfected with miR-192-5p mimic. **p < 0.01, ****p < 0.0001 (E–F) The expression of LC3, p62, α-SMA, Col I, and FN in HK-2 and NRK-49F cells transfected with miR-192-5p mimic (G) The cell viability in NRK-49F cells transfected with miR-192-5p mimic. ****p < 0.0001 (H) HK-2 cells were transfected with miR-192-5p mimic and treated with rapamycin, the expression of LC3, p62, Col I, and FN was detected (I) Relative expression of miR-192-5p in HK-2 cells treated with HG and DHT. **p < 0.01 (J–K) Relative expression of miR-192-5p in HK-2 and NRK-49F cells transfected with miR-192-5p mimic and treated with DHT. *p < 0.05, ****p < 0.0001 (L–O) The expression of LC3, p62, ATG5, α-SMA, Col I, and FN in HK-2 and NRK-49F cells transfected with miR-192-5p mimic and treated with DHT was determined by Western blot and the relative intensity of LC3-II was analyzed by the ChemiScope analysis software (*p < 0.05, **p < 0.01, ****p < 0.0001).