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. 2021 Jul 19;12:711158. doi: 10.3389/fmicb.2021.711158

FIGURE 2.

FIGURE 2

Intercistronic region of tetR and tetA(G). (A) The A. baumannii AYE intercistronic region of tetR-tetA(G) was analyzed for operator sequences using the EMBOSS palindromes prediction tool (Rice et al., 2000). The intergenic sequence located between tetR and tetA(G) genes contains two inverted repeat (IR) sequences, IR1 and IR2, highlighted in yellow. The divergently orientated genes tetR and tetA(G) are indicated by arrows and highlighted in green. (B) Schematic representation of the amplified dsDNA of the intercistronic region between tetR and tetA(G) genes of A. baumannii AYE. PCR products of different length consisting IR1 and/or IR2 (319–65 bp) were amplified from genomic DNA of A. baumannii AYE. The starts of the open reading frames of the tetR and tetA(G) genes are indicated in yellow and green, respectively. The intergenic region is indicated in blue and the IRs are highlighted in red. (C) Binding of dsDNA containing IR1 and/or IR2 to TetR in the electrophoretic mobility shift assay.