FIGURE 6.

Minocycline (MIN) binding induced conformational changes of AbTetR. (A) Conformational changes of the DNA binding domain of the unliganded AbTetR and the liganded Gln116Ala structures. Structural superimposition was performed based on the main chain atoms of helices α8 and α10 (Yu et al., 2010). (AbTetR-A: black, AbTetR-B: blue, Gln116Ala-A: green, Gln116Ala-B: cyan, Gln116Ala-C: magenta, Gln116Ala-D: yellow). (B) Water-mediated stabilization of the β-turn upon minocycline binding. Water molecules and the Mg²⁺ are depicted as red and green spheres, respectively.