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. 2021 Mar 22;7(3):609–620. doi: 10.1016/j.aninu.2020.11.008

Table 3.

Effects of XOS on mRNA expression of key genes of inflammatory signaling pathways in the small intestine of piglets after LPS challenge at 4 h postinjection.1

Item Saline
 LPS
 SEM P-value
CON XOS CON XOS Diet LPS Interaction
Jejunum
 TLR4 1.00 0.67 1.36 1.24 0.10 0.031 <0.001 0.303
 MyD88 1.00 0.81 0.93 0.91 0.07 0.173 0.860 0.249
 IRAK1 1.00 0.81 0.94 0.95 0.07 0.256 0.596 0.169
 TRAF6 1.00b 0.69a 1.02b 1.01b 0.05 0.009 0.005 0.012
 NOD1 1.00 0.72 1.26 0.98 0.12 0.032 0.044 0.995
 NOD2 1.00 0.86 2.74 1.36 0.61 0.251 0.001 0.857
 RIP2 1.00b 0.62a 1.45c 1.59c 0.07 0.099 <0.001 0.001
 NF-кB 1.00bc 0.86a 1.01bc 1.25c 0.09 0.572 0.044 0.054
 TNF-α 1.00 0.73 0.95 0.52 0.15 0.034 0.384 0.614
 IL-6 1.00 1.06 1.35 1.60 0.13 0.235 0.002 0.454
 COX2 1.00 0.65 1.48 1.44 0.15 0.197 <0.001 0.306
 HSP70 1.00a 0.46a 4.01a 18.93b 1.00 0.006 0.016 0.004
Ileum
 TLR4 1.00 0.93 1.39 1.03 0.09 0.032 0.015 0.127
 MyD88 1.00 0.82 0.89 0.80 0.06 0.037 0.256 0.447
 IRAK1 1.00 0.97 0.97 0.84 0.06 0.231 0.184 0.422
 TRAF6 1.00 0.88 0.90 0.83 0.07 0.182 0.302 0.757
 NOD1 1.00 0.52 0.91 0.55 0.12 0.003 0.817 0.640
 NOD2 1.00 0.76 1.20 1.05 0.09 0.048 0.015 0.632
 RIP2 1.00 0.99 1.32 1.16 0.09 0.402 0.018 0.442
 NF-кB 1.00b 0.96b 1.04b 0.82a 0.03 0.001 0.150 0.019
 TNF-α 1.00 0.95 1.04 0.72 0.09 0.046 0.283 0.129
 IL-6 1.00b 0.69a 0.76a 0.72a 0.07 0.020 0.143 0.068
 COX2 1.00 0.65 0.90 0.75 0.10 0.024 0.981 0.359
 HSP70 1.00a 0.93a 12.53b 31.07c 3.00 0.006 <0.001 0.006

CON = control; XOS = xylooligosaccharide; LPS = lipopolysaccharide; TLR4 = Toll-like receptor 4; MyD88 = myeloid differentiation factor 88; IRAK1 = IL-1 receptor-associated kinase 1; TRAF6 = TNF receptor associated factor 6; NOD = nucleotide binding oligomerization domain protein; RIP2 = receptor-interacting serine/threonine-protein kinase 2; NF-кB = nuclear factor-κB; TNF-α = tumor necrosis factor-α; IL-6 = interleukin-6; COX2 = cyclooxygenase 2; HSP70 = heat shock protein 70.

a, b, c Labeled means in a row without a common letter differ at P < 0.05.

1

Values are means and pooled SEM, n = 6 (1 pig/pen).