HFD induces Nprc expression and PPARγ enhancer activity in adipose tissue.A, levels of Nprc mRNA and the −49 kb PPARγ enhancer RNAs (eNprc-49k) in brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT) of wildtype mice fed with control diet (CD) or HFD. B, levels of Fabp4 and the −5.4 kb PPARγ enhancer RNA (eFabp4-5.4kb) in BAT and iWAT of mice fed with CD and HFD. C, levels of Npra mRNA in the BAT and iWAT of mice fed with CD and HFD. Student's t test, ∗p < 0.05 and ∗∗∗∗p < 0.0001. Fabp4, fatty acid–binding protein 4; HFD, high-fat diet; Npra, NP receptor A; Nprc, NP receptor C; ns, not significant; PPARγ, peroxisome proliferator–activated receptor gamma.