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. 2021 Jul 19;9:653808. doi: 10.3389/fcell.2021.653808

FIGURE 8.

FIGURE 8

Long non-coding RNA (lncRNA) KCNQ1OT1 regulates tumor cell immune escape via miR-30a-5p/ubiquitin-specific peptidase 22 (USP22) in the in vitro assay. (A) The expression of miR-30a-5p in patient samples. (B) The correlation between miR-30a-5p and lncRNA KCNQ1OT1 in tumor tissues. (C) Expression of miR-30a-5p in different types of colorectal cancer (CRC) cells. (D,E). Dual-luciferase report and ribonucleoprotein immunoprecipitation (RIP) assay verify the targeting of lncRNA KCNQ1OT1 and miR-30a-5p. The binding site prediction comes from the Starbase database. (F) Dual-luciferase report verifies the targeting of USP22 and miR-30a-5p. The binding site prediction comes from the Starbase database. (G) Activated T cell and SW1463 cells were cocultured in six-well plates for 7 days, and then surviving SW1463 cells were visualized by colony formation assay. (H,I) T cell-mediated SW1463 cell killing analysis measured by Cell Counting Kit (CCK)-8 assay and detection of apoptotic protein. (J,K) Programmed death-ligand 1 (PD-L1) protein expression of SW1463 cells in different treatment groups. ∗p < 0.05 compared with the relative control group.