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. 2021 Jul 19;11:711144. doi: 10.3389/fcimb.2021.711144

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Copyright © 2021 Murase, Aikawa, Nozawa, Nakatake, Sakamoto, Kikuchi and Nakagawa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of proteome profiles of EVs from S. pyogenes str. SSI-1 and JRS4. (A) Heatmap representation of clustering according to presence and absence of proteins in EV from SSI-1 and JRS4, with biological triplicates. (B) Venn diagram of EVs-derived proteins identified in SSI-1 and JRS4. (C) Heatmap representation of clustering according to log10-transformed abundance values of common proteins identified in two strains. Arrows indicate well-known virulence factors in GAS. (D) Abundance ratio significantly higher (> 2.0) in SSI-1 or JRS4 that are shown in bar-chart. Heatmap generation was implemented in R by using heatmap.2 from ggplots package.