FIGURE 1.

Migration trajectories of the three species under external experimental conditions. Panels (A) show the migration without any stimuli by each of the three species (A. proteus, M. leningradensis, and A. borokensis, respectively). Practically, the amoebae explored all the directions of the experimentation chamber. In addition to the trajectories, the approximated percentage of cells that displaced in certain portion of the exploration chamber is represented. The space has been divided in 8 sections (thus π/4 angle amplitude was used for determining the regions). In panels (B), the locomotion trajectories during galvanotaxis are depicted (96% of A. proteus, 97% of M. leningradensis, and 94% of A. borokensis migrated toward the cathode). Next to the trajectories, the respective histograms of the displacement cosines are illustrated. Finally, panels (C) represent the migration during chemotaxis. 76%, 76% and 73% of the respective cells migrated toward the chemotactic gradient. Alongside the trajectories, the histograms for the angles of each trajectory have been illustrated. “N” is the total number of cells, “Er” is the experimental replications, “nr” is the number of cells per replication, “t” time of galvanotaxis or chemotaxis, “p” chemotactic peptide (nFMLP), “+” anode, “−” cathode. Both the x and y-axis show the distance in mm, and the initial location of each cell has been placed at the center of the diagram.