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. 2021 Jul 3;297(2):100937. doi: 10.1016/j.jbc.2021.100937

Figure 3.

Figure 3

Epistasis analysis of known ER-shaping proteins and NOMO1.A, U2OS cells were treated with respective siRNA for 48 h and stained with PDI as an ER marker. B, representative image of Atl2-FLAG overexpression (left panel) rescuing the NOMO KD phenotype as judged by PDI staining (right panel). C, quantification of the ability of ER-shaping proteins to rescue the NOMO KD ER phenotype, n = 100, N = 4. Error bars indicate SD. ∗∗∗p < 0.002; ∗∗∗∗p < 0.0001. D, representative image of FLAG-NOMO1r overexpression (left panel) rescuing Atl2 KD phenotype (right panel). E, quantification of NOMO1 and Climp63 overexpression rescuing the Atl2 KD phenotype, n = 100, N = 3. Error bars indicate SD. ∗∗p < 0.01. Scale bars are 10 μm. ER, endoplasmic reticulum; NOMO1, nodal modulator 1; ns, not significant; PDI, protein disulfide isomerase.