Single-particle analysis of NOMO1.A, top-10 two-dimensional class averages of ∼7000 picked negative-stain NOMO1-FLAG particles, numbers of particles per class in squares. Mask diameter is 40 nm. A construct layout is inserted to clarify protein domains. B, three-dimensional reconstruction from panel A; the scale bar is 10 nm. C, top-10 two-dimensional class averages of ∼10,000 picked negative-stain NOMOLD-FLAG particles, the number of particles per class in squares. Mask diameter is 30 nm. A construct layout is inserted. D, three-dimensional reconstruction from panel C, the scale bar is 10 nm. CYT, cytosolic tail; NOMO, nodal modulator; TM, transmembrane domain.