FIGURE 3.
Phylogenetic analysis of Sideroflexin family. Phylogenetic reconstruction is the result of Bayesian inference posterior probabilities and bootstrapping analysis with the best-scoring maximum likelihood tree using RAxML (v8.2.10) (Minjarez et al., 2016) on 30 taxa with 67 sequences. Support values are given in percent at the nodes of the major clades differentiating the sideroflexin gene families. The protein sequences were aligned using Mafft (v6) (Fang et al., 2018) with E-INSI-I algorithm and JTT substitution model. MrBayes was used with amino acid mixed model run for 1,000,000 generations. The PROTGAMMAAUTO model in RAxML was used with 500 bootstrap replicates.
