Fig. 6.

Role of miR-144-5p in BMSCs osteogenesis differentiation. a Levels of miR-144-5p in BMSCs transfected with mimic-NC, miR-144-5p-mimic, inhibitor-NC or miR-144-5p-inhibitor were analyzed by qRT-PCR. b–d The osteogenic genes at the mRNA and protein levels in the transfected BMSCs were confirmed via qRT-PCR and Western blotting. e The proportion of mineralization in the transfected BMSCs was shown by Alizarin red staining and ALP staining assays. f, g The statistical data of Alizarin red staining and ALP staining. Data are presented as the mean ± SD, and all experiments were repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001