Table 2.

Limit of detection at 95 and 50% confidence interval (LOD_95% and LOD_50%) for SARS-CoV-2 and surrogates PEDV and E229 on glass, steel and plastic surfaces following the selected sampling Method A coupled with MAX RNA extraction procedure.

Inoculated virus		Glass			Steel			Plastic
		Positivesa	LOD95%b	LOD50%b	Positives	LOD95%	LOD50%	Positives	LOD95%	LOD50%
SARS-CoV-2 (gc/4 cm2)	5.10 × 104	6/6	3.31 × 102	7.65 × 101	6/6	2.18 × 102	5.03 × 101	6/6	6.37 × 102	1.47 × 102
	5.10 × 103	6/6			6/6			6/6
	5.10 × 102	6/6			6/6			6/6
	5.10 × 101	2/6			3/6			0/6
	5.10 × 100	0/6			0/6			0/6
E229 (PCRU/4 cm2)	2.28 × 105	6/6	1.60 × 103	3.70 × 102	6/6	4.92 × 103	1.14 × 103	6/6	4.65 × 103	1.08 × 103
	2.28 × 104	6/6			6/6			6/6
	2.28 × 103	6/6			5/6			4/6
	2.28 × 102	2/6			0/6			2/6
	2.28 × 101	0/6			0/6			0/6
PEDV (PCRU/4 cm2)	1.42 × 104	6/6	3.60 × 102	8.34 × 101	6/6	6.94 × 102	1.61 × 102	6/6	1.01 × 103	2.33 × 102
	1.42 × 103	6/6			6/6			6/6
	1.42 × 102	4/6			3/6			2/6
	1.42 × 101	1/6			0/6			0/6
	1.42 × 100	0/6			0/6			0/6

^aVirus positive/total number of samples.

^bCalculated according to Wilrich and Wilrich (2009).