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. 2021 Jul 22;2(3):100682. doi: 10.1016/j.xpro.2021.100682

Buffer B

Reagent Final concentration Amount
TFA 0.1% (v/v) 0.05 mL
Acetonitrile 80% (v/v) 40 mL
Ultrapure water n/a 9.95 mL
Total n/a 50 mL

The solution is prepared just before use.