N-Loading buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| FA | 2.5% (v/v) | 0.125 mL |
| Acetonitrile | 30% (v/v) | 1.5 mL |
| Ultrapure water | n/a | 3.375 mL |
| Total | n/a | 5 mL |
The solution is prepared just before use.
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