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. 2021 Jul 22;2(3):100682. doi: 10.1016/j.xpro.2021.100682

C-Loading buffer

Reagent Final concentration Amount
TFA (10%) 0.15% (v/v) 0.075 mL
Acetonitrile 30% (v/v) 1.5 mL
Ultrapure water n/a 3.425 mL
Total n/a 5 mL

The solution is prepared just before use.