C-0.5% TFA buffer

Reagent	Final concentration	Amount
TFA (10%)	0.5% (v/v)	0.25 mL
Acetonitrile	30% (v/v)	1.5 mL
Ultrapure water	n/a	3.25 mL
Total	n/a	5 mL

The solution is prepared just before use.