Other solutions

Reagent	Note
HEPES-NaOH, pH 8.5 (200 mM)	Adjust the pH to 8.5 with NaOH. The solution can be stored at 4°C for two weeks.
BB-94 (20 mM)	Dissolved in DMSO. The solution can be stored at –20°C for three months.
PMA (5 mM)	Dissolved in DMSO. The solution can be stored at –20°C for three months.
EDTA-NaOH, pH 8.0 (200 mM)	Adjust the pH to 8.0 with NaOH. The solution can be stored at 25°C for three months.
EGTA-NaOH, pH 8.0 (200 mM)	Adjust the pH to 8.0 with NaOH. The solution can be stored at 25°C for three months.
PMSF (100 mM)	Dissolved in DMSO just before use.
Hydroxylamine (1%)	Hydroxylamine solution (wt. 50%) is 50-fold-diluted with ultrapure water. The solution can be stored at 25°C for two weeks.
CaCl2 (10 mM)	The solution can be stored at 25°C for three months.
Ammonium bicarbonate (50 mM)	The solution can be stored at 4°C for three months.