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. 2020 Jul 16;106(8):2147–2160. doi: 10.3324/haematol.2020.253716

Figure 2.

Figure 2.

Visualization of acute graft-versus-host disease- associated ultrastructural changes in the liver by transmission electron microscopy. Representative pictures of sections from liver taken at day+15 after experimental hematopoietic stem cell transplantation (HSCT) in the chemotherapy based B6→BDF model. (A and B) Liver sinusoidal endothelial monolayer after syngeneic-HSCT (syn-HSCT) without acute graft-versus- host disease (aGvHD). (A) Normal, fenestrated sinusoidal blood vessel completely covered with endothelial monolayer. (B) Higher magnification of a 100 nm large fenestration of the endothelium in the liver. (C to-F) Sinusoidal liver endothelial monolayer after allo-HSCT during aGvHD. (C) Liver sinusoidal vessel with destroyed and unregularly shaped endothelial monolayer in contact with an immune cell. (D) Higher magnification of contact zone between immune cell and endothelial cell. (E) Blistering of the endothelial monolayer with a platelet in the region of injury. (F) Higher magnification of endothelial blistering. The perivascular space is marked by a red triangle. V: vessel lumen; EM: endothelial monolayer; F: fenestrated endothelium; IC: immune cell; E: erythrocyte; P: platelet; red circle: loss of endothelium; red triangle: endothelial blistering. Control groups (no aGvHD) were transplanted with the same bone marrow cell numbers and T-cell numbers from syngeneic donors.