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. Author manuscript; available in PMC: 2021 Aug 2.
Published in final edited form as: Cell Rep. 2021 Jun 22;35(12):109269. doi: 10.1016/j.celrep.2021.109269

Figure 3. Genes mis-regulated in Fezf2−/− cortices were enriched in projection neuron subtype-specific genes.

Figure 3.

(A) Left heatmap shows normalized gene expression levels in P0 Fezf2−/− (n = 4), Fezf2−/−; Fezf2-EnR (n = 2), and control Fezf2+/+ (n = 3) cortices. The subtype specificities for the mis-regulated genes were determined based on the DeCoN dataset. CPN, callosal projection neuronal genes; CThPN, corticothalamic neuronal genes; SCPN, layer 5 subcerebral projection neuronal genes; mixed identity, genes expressed in more than one neuronal subtype; cell-type independent, genes expressed in all subtypes.

(B and C) In situ hybridization and RNA-seq analyses showed reduced expressions for Ephb1, Tcerg1l, Ldb2, Wnt7b, Kif26a, and Ctgf and increased expression of Cryab in Fezf2−/− cortices, which were restored in the Fezf2−/−; Fezf2-EnR mice. Bar graphs showed normalized mRNA expression levels detected by RNA-seq in the cortices for the P0 Fezf2+/+ (n = 3 mice), Fezf2 −/− (n = 4 mice), and Fezf2−/−; Fezf2-EnR (n = 2 mice) mice. Error bars represent SEM. Scale bars: 500 μm in (B) and 200 μm in (C).