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. Author manuscript; available in PMC: 2021 Aug 2.
Published in final edited form as: Cell Rep. 2021 Jun 22;35(12):109269. doi: 10.1016/j.celrep.2021.109269

Figure 4. TLE4 and FEZF2 can bind to each other and are co-expressed in the corticothalamic neurons.

Figure 4.

(A) Co-immunoprecipitation experiment showed that mouse FEZF2 and TLE4 proteins can bind each other. E, elution; FT, flow through; In, input. The bands around 50 KD are FEZF2-HIS-MYC; the bands around 75 KD are TLE4-FLAG-MYC.

(B) Immunostaining for TLE4 and FEZF2 on brain sections from E14.5, E16.5, and P0 in wild-type mice. Low-mag single z-plane image scale bar: 50 μm; high-mag maximum z-projection image scale bar: 10 μm.

(C) Combined retrograde tracing and immunostaining show that TLE4 is expressed in >99% of retrogradely labeled corticothalamic neurons in M1 (1,034 TLE4+CTB+ among 1,050 CTB+ cells), 0% of the traced subcerebral projection neurons (930 cells), and 0.1% of the callosal projection neurons (2 TLE4+CTB+ among 1,783 CTB+ cells). n = 3 mice for each injection location and 3 sections per brain were quantified. Low-mag scale bars: 1,000 μm. Scale bars in the second column from the right: 100 μm. Scale bars in the rightmost column: 20 μm.