Figure 3.

Accessible chromatin is localized in narrow peaks around the Iso-Seq TSSs. We compared ATAC-seq read coverage at all Ensembl TSSs and Iso-Seq TSSs across the autosomes in the male liver (A) and female liver (B). ATAC-seq reads show an enrichment at the Iso-Seq TSS compared with the Ensembl TSS. This indicates a more accurate positioning of the TSS using Iso-Seq. A second male and female replicate is shown in Supplemental Figure S5.