Fig. 4.

IHLLS 2L beads volume reconstruction; a) 500 nm beads holography $\pm 40\mu m,\pm 30\mu m$, holograms (first row), phase maps (second row), and reconstructed images (third row); b) The xy and yz cross-sections of a bead after the hologram reconstruction together with the z-max projection of all of the best z-reconstructed planes. The IHLLS phase images contain the depth dependent phase information derived from the IHLLS holograms and the reconstructed IHLLS images show the complex holograms propagated to the best focal plane. The max projection of the reconstructed volume of the 500 nm beads sample contains the z-galvo levels $\pm 40\mu m,\pm 30\mu m,$ $\pm 20\mu m,\text{and}0\mu m$. The transverse MTFs of the two beads b#1 and b#2 are shown in (c, d) and the axial MTFs are shown in (e, f); g) 1D xy and yz sections of the MTFs, h) 1D xy and yz of the PSFs. The FWHM of the curves are blue- 0.4534 µm, red 0.5118 µm, green 0.7663 µm, and black 0.7946 µm.