Figure 3. N‐glycosylation at position 83 is required and sufficient for ATZ delivery to endolysosomes.

• A
  ATZ glycosylation mutants and their electrophoretic mobility. N is the acceptor site asparagine; Q is for the mutation to glutamine that prevents glycosylation. The E342K mutation of ATZ_NNN is in blue.
• B–I
  Confocal laser scanning microscopy analyses (as in Fig 2) in WT MEF treated with BafA1.
• J, K
  (J) Quantification of ATZ_xxx‐positive endolysosomes and (K) of ATZ_xxx polymers‐positive endolysosomes (mean, n = 18, 30, 24, 14, 27, 22, 27, 18 cells for HA stain and n = 12, 14, 14, 11, 14, 14, 17, 11 cells for 2C1 stain of panels B‐I, respectively). One‐way ANOVA and Dunnett’s multiple comparison test, ns P > 0.05, ****P < 0.0001.

Data information: Scale bars 10 μm.

Source data are available online for this figure.